南京M5144-TP FastAmpli RT Premix- UNG (Probe qRT- PCR)

Product Code: M5144-TP

Recommended Applications:

For sexually transmitted infection (STI) detection

Product Introduction:

FastAmpli RT Premix-UNG (Probe qRT-PCR) is a specialized reagent for fluorescent quantitative (TaqMan probe method) RNA amplification detection, containing reverse transcriptase and DNA polymerase that have been genetically modified for rapid amplification. The PCR reaction can be completed within 20-40 minutes. This product has strong tolerance to inhibitors and higher reverse transcription and PCR amplification efficiency, making it suitable for high-sensitivity amplification of low-concentration RNA templates. It can obtain good standard curves in a wide range of quantitation areas, allowing accurate quantitation. This reagent uses a mixed enzyme of anti-inhibitory amplification enzyme and UNG enzyme, as well as an optimized buffer system containing dUTP, which not only enables good amplification of the target gene in samples with inhibitors but also effectively prevents false positive reactions caused by residual PCR products or aerosol contamination.

Composition of the Premix

1. 10×FastAmpli RTase/UNG Mix

2. 5×FastAmpli RT Premix Buffer (dUTP) (Mg2+ free) (DG)

3. 25×MgCl2

Reaction Setup:

for Regular PCR:

Reverse Transcription: at 50 ℃ for 10 to 20 mins

Denaturation: at 95℃ for 1 to 5 mins

Denaturation: at 95℃ for 10 to 20 sec

Annealing and Extension: at 56~64℃ for 20~60 sec

for Rapid PCR:

Reverse Transcription: at 50 ℃ for 5 mins

Denaturation: at 95℃ for 30 sec

Denaturation: at 95℃ for 1 to 3 sec

Annealing and Extension: at 56~64℃ for 3 to 20 sec