淮安HW206-M01 Colorimetric RT-LAMP Premix (RY)

Product Code: HW206-M01

Intended Use

This kit uses visual isothermal amplification for the qualitative detection of DNA or RNA in samples such as viruses.

Product Introduction

This kit employs LAMP technology, using 4-6 specific LAMP primers designed for the target sequence. The Bst2.0 HS isothermal amplification enzyme and specific primers recognize 6-8 independent regions of the target sequence, generating double-stranded DNA through isothermal amplification. The amplification process produces pyrophosphate, lowering the pH value of the reaction, which can be observed by a color change in the pH indicator. Before the reaction, the mixture is purplish-red; after a positive reaction, it turns yellow, while a negative reaction remains purplish-red.

Product Specification:

Reaction Temperature: 60~65℃，30~60min (After the reaction is completed, the results should be interpreted after cooling to room temperature.) 

Lyophilization: Yes

Reagent Compositions

1. Bst2.0 HS (8 U/µL)

2. 2×RT-LAMP Premix Buffer (RY)

3. Neoscript RTase (200 U/μL)