北京HW205-M01 Colorimetric LAMP Premix (RY)

Product Code: HW205-M01

Intended Use

This kit uses a dye-based isothermal amplification, which can be used for qualitative detection of DNA in samples (such as viruses).

Product Introduction

This reagent kit uses LAMP technology and is designed to target the specific sequence with 4-6 specific LAMP primers. It utilizes Bst 2.0 HS isothermal amplification enzyme to recognize 6-8 independent regions of the target sequence, generating double-stranded DNA through isothermal amplification. During the amplification process, pyrophosphate ions are produced, leading to a decrease in pH. Therefore, the color change of the pH indicator can be used to interpret the results. The reagent kit appears purple-red before the reaction, with the positive wells turning yellow and the negative wells maintaining the purple-red color after the reaction.

Product Specification:

Reaction Temperature: 60~65℃，30~60min (After the reaction is completed, the results should be interpreted after cooling to room temperature.) 

Lyophilization: Yes

Reagent Compositions

1、Bst2.0 HS (8 U/µL)

2、2×LAMP Premix Buffer (RY)