E01 Uracil-DNA Glycosylase

Product Code: E01

Product Introduction:

Uracil-DNA Glycosylase (UNG or UDG) is derived from recombinant cloning and expression of Escherichia coli, with a molecular weight of 

25 kDa. It can catalyze the release of free uracil in single-stranded and double-stranded DNA containing uracil, and has no activity on RNA.

 It can be used to prevent contamination caused by PCR amplification products. Its principle is based on: if dUTP is substituted for dTTP in 

PCR reaction to form PCR amplification products containing dU bases, this enzyme can selectively break the glycosidic bond of U bases in 

single-stranded and double-stranded DNA, degrading the PCR amplification product.

Product Specification:

Concentration: 1U/μL

Lyophilization: Yes

Storage Buffer:

20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, Stabilizer, 50% Glycerol.

Features:

- Digest dUTP template at 50℃

- Inactivated when heated at 95°C